Fig. 1.

Heat-shocked HT1080 cells display a unique HSF1 foci on chromosome Y. a Unique SATIII RNA foci are observed in the nucleus of cell lines of male origin (bar = 5 μm). SATIII RNA (red signals) are detected by RNA FISH with a fluorescent oligonucleotide recognizing (GGAAU)n motifs, on male cells of different origin (HT1080 cells, amniocytes and primary fibroblasts). b HSF1 detected by immuno-fluorescence in HT1080 cells colocalizes with the Yq12 locus and not with the 9q12 locus detected by DNA FISH. Upper image Yq12 locus (red signals), HSF1 (green signal) (bar = 5 μm). Lower image 9q12 locus (green signal), HSF1 (red signal) (bar = 5 μm). c Graph giving the percentage of nSBs present on chromosome 9, chromosome Y, or both, in normal fibroblasts. HSF1 foci were codetected by immunofluorescence and FISH in human primary stressed male and female fibroblasts (total of 200 nuclei analyzed on three independent analyses)