Figure 5.

Cursor tuning shifts to target location

(A) Electrophysiological recordings were targeted to AM while animals were performing the imaging-based BMI task, with control regions in the anterior motor cortex. Inset shows example waveforms from 3 isolated units (fast spiking unit in red).

(B) Example spiking during 2 successful trials (trials denoted in blue, hits denoted with pink arrows). At top, traces, from top to bottom, are pupil diameter, running velocity, and visual cursor position.

(C) Normalized tuning to cursor positions for all single units. Each row represents the normalized firing responses to each of 8 cursor positions for every recorded unit in the task (top, N = 131 units) and playback (bottom, N = 128 units) conditions. Firing responses were taken as the average firing rate for a period from 80 to 200 ms from the onset of the cursor presentation.

(D) Average population firing rates for each cursor position during task performance (red) and passive playback (blue). Shaded regions indicate 95% confidence levels.

(E) Left: mean firing rate for regular spiking (RS) units to different cursor positions during task performance (95% confidence interval indicated by shading, n = 7 mice). Right: mean firing rate for fast spiking (FS) units to different cursor positions during task performance (95% confidence interval indicated by shading, n = 7 mice).