TABLE 1.
Primer/Probe | Sequence (5′-3′) | Genomic position | Product size (bp) |
RT-RPA-F | [Biotin]GGAGTTGTAGAGGTRAAGGACTCGGGYACTA | 259–289 | – |
RT-RPA-R | TCCGGCTTAGGGGCYCCTGGTGGYACATACAT | 445–476 | 218 |
RT-RPA-P | [FAM]AGGTTGGACACAAAAGTGAACTCRGCATCAA[dSpacer]GCGCATGTATGTTGA[C3-spacer] | 361–407 | 149 |
FAM: Carboxyfluorescein; dSpacer: A tetrahydrofuran residue; C3-spacer, 3′-block.