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. 2021 Feb 4;12:629533. doi: 10.3389/fmicb.2021.629533

TABLE 1.

Primer and probe sequences used for RT-RPA-LFS.

Primer/Probe Sequence (5′-3′) Genomic position Product size (bp)
RT-RPA-F [Biotin]GGAGTTGTAGAGGTRAAGGACTCGGGYACTA 259–289
RT-RPA-R TCCGGCTTAGGGGCYCCTGGTGGYACATACAT 445–476 218
RT-RPA-P [FAM]AGGTTGGACACAAAAGTGAACTCRGCATCAA[dSpacer]GCGCATGTATGTTGA[C3-spacer] 361–407 149

FAM: Carboxyfluorescein; dSpacer: A tetrahydrofuran residue; C3-spacer, 3′-block.