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. 2021 Feb 17;12:1096. doi: 10.1038/s41467-021-21346-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Workflow of tissue preparation for single-cell transcriptome profiling of human thymic stromal cells. CD45-negative cells were enriched using magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS). b Stacked bar graph of cell-type frequencies in each sample. Source data are provided as a Source Data file. c UMAP visualization of thymic stromal cells colored by age group. d UMAP visualization of thymic stromal cells colored by cell types. e UMAP visualization of the expression of known marker genes used for cell cluster identification. f Heatmap showing average expression of soluble factors, extracellular matrix/adhesion molecules, and chemokines in each stromal cluster. g Violin plots showing feature gene expression in human fetal thymus (hFT), human postnatal thymus (hPT) or adult thymus. Colors represent age group. h Immunofluorescence analysis of fibronectin (FN) and K15⁺ immature TECs/mTECs (green) expression in human fetal thymus (hFT). Arrows point to blood vessels with high expression of FN in the cortex (c) and medulla (m). Scale bar, 50 μm. Staining was repeated twice with similar results. i UMAP and violin plots showing expression of the activin ligand (INHBA) and inhibitor (FST) in human fetal thymus (hFT), human postnatal thymus (hPT) or adult thymus. Colors represent age group.