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. 2021 Feb 17;12:1096. doi: 10.1038/s41467-021-21346-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a UMAP visualization of epithelial cells colored by age group. b UMAP visualization of epithelial cells colored by cell types. c UMAP visualization of the expression of known marker genes used for cell cluster identification. d Heatmap showing the average expression of known marker genes in each epithelial cluster. e UMAP visualization of epithelial subsets in fetal, postnatal, and adult samples. f Immunofluorescence staining of AIRE⁺ (green) K5⁺ (red) mTEC^hi cells in postnatal and adult tissues. Arrowheads point to AIRE⁺ cells. Scale bars, 50 μm. g Thymic tissue from a 13-year-old donor was stained with CD8 (green) and CD4 (red) antibodies to visualize thymocytes together with a wide spectrum cytokeratin antibody to identify epithelial cells (blue). Scale bars, 50 μm. Staining in f and g was repeated twice with many donors with similar results.