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. 2021 Feb 17;12:1096. doi: 10.1038/s41467-021-21346-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Velocity field projected on the UMAP plots of fetal, postnatal, and adult samples. b Dot plot depicting the relative level of expression of Notch signaling ligands, receptors, target genes, and inhibitors in epithelial subsets. c Immunofluorescence staining of human fetal thymus showing expression of HES1 (red) in medullary KRT15+ TECs (green). Scale bar, 100 μm. Staining was repeated twice with similar results. d–f Dot plots depicting the relative level of expression of selected TNF Superfamily (d), p53 (e), or Toll-like receptor signaling pathway (f) genes.