Table 1.
List of target genes and primer sequences for qPCR.
| Accession no. | Gene product name | Primers (5′–3′) Forward Reverse |
Length [bp] | Tm [°C] | Efficiency (%) | R2 |
|---|---|---|---|---|---|---|
| TERG_07193 | Kynurenine aminotransferase | GCAAGCAACGAGCCTTTCAA | 196 | 60.5 | 101 | 0.99814 |
| GGTTAGACTCCACGGTCTGC | ||||||
| TERG_04125 | Citrate synthase | GGCTCCTACCCTCAAGGAGA | 195 | 60.5 | 100 | 0.99755 |
| CGGAAGCGAATACCCTCCTC | ||||||
| TERG_01612 | Enolase | GAAGGGTGTCCCACTGTACG | 132 | 60.5 | 97 | 0.99675 |
| AACTCCTGGAAAGCGAGACG | ||||||
| TERG_00247 | Riboflavin synthase | GGGCGGGACATCCTTAACAA | 190 | 60.5 | 102 | 0.99855 |
| GACTGGTGATCCGGCTTTGA | ||||||
| MCYG_08415 | SSU1 gene | AAGAGCTTCAGGTCACAGCC | 191 | 60.5 | 99 | 0.99655 |
| AAGCCCGGGAAACTGGTATG | ||||||
| MCYG_06837 | Citrate synthase | GACACAAGGTCCTCGGTGAG | 146 | 60.5 | 108 | 0.99723 |
| GAGTTTCTGGCACTCGGGAA | ||||||
| MCYG_07208 | Enolase | CGGTGTCAGTTTGGCCATTG | 171 | 60.5 | 101 | 0.99651 |
| AACTCCTGGAAGGCAAGACG | ||||||
| MCYG_00702 | Riboflavin synthase | AGCTCCTTTCAGTCACACCG | 113 | 60.5 | 98 | 0.99490 |
| AGTGCCGTCAAGAGCAATGA | ||||||
| XM_002848521 | ADP ribosylation factor (adp-rf) | GAATTCTCATGGTCGGTCTC | 104 | 60.5 | 100 | 0.99855 |
| AACGTTGAATCCGATGGTG | ||||||
| XM_002843632 | Multiubiquitin chain binding protein 1 (mbp1) | AGTCCTAGTTACCTTGACCG | 123 | 60.5 | 99 | 0.99924 |
| CGGTGTTTAAGTGCTAGATAGG | ||||||
| TERG_00548 | Elongation factor 1-alpha (ef1-α) | GAGAAGTTCGAGAAGGAAGC | 128 | 60.5 | 107 | 0.99803 |
| TGACGGTGACATTGTACTTG | ||||||
| TERG_04033 | Ribosomal protein L2 (rpl2) | GGATCTATATTCACGGCTCG | 113 | 60.5 | 102 | 0.99484 |
| TGGATGATGTTCTTCACGAC |