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. 2021 Feb 17;6:27. doi: 10.1038/s41541-021-00288-6

Fig. 1. Characterization of ZE4B-36 replication in cell culture.

Fig. 1

a Schematic of construction of ZIKV FSS13025 mutant virus. Restriction enzyme sites used for cloning are indicated. b IFA of viral protein expression in Vero cells transfected with WT FSS13025ic (WT) and ZE4B-36 RNAs. Vero cells were electroporated with 10 μg genome-length WT and ZE4B-36 RNAs RNA. On days 2 and 3 post transfection, IFA was performed to examine viral E protein expression using a mouse mAb (4G2). c Plaque morphologies of WT and ZE4B-36 viruses. Plaques were developed on a Vero cell monolayer after 4 days of infection. d Comparison of growth kinetics of WT FSS13025ic and ZE4B-36 viruses in Vero cells. Cells were infected with both viruses at an MOI of 0.01. Viral titers were measured at the indicated time points using plaque assays on Vero cells, n = 3. Means and SDs from three independent replicates are shown. e, f A549 cells were infected with WT FSS13025ic or ZE4B-36 virus at an MOI of 0.1. At days 1 and 4 pi, viral load was measured by Q-PCR (e) and FFA (f). n = 4 to 8. Data are presented as means ± standard error of the mean (s.e.m). ***P < 0.001 or **P < 0.01 compared to WT group.