Extended Data Fig. 1 |.
Cryo-EM structural analysis of the native NSD3C bound to 187-bp NCP. a, A representative cryo-EM micrograph from a total 2511 micrographs of the NCP-NSD3C complex (low-pass filtered to 20 Å). Scale bar, 100 nm. b, Selected 2D class averages of the NCP-NSD3C complex. Scale bar, 10 nm. Box size 216, pixel size 1.08 Å. c, Workflow of the NCP-NSD3C complex image processing procedures. It includes several rounds of 2D and 3D classification, refinement and masked refinement. The linker DNA is highly flexible, thus, it is invisible when the density map was shown in high resolution. d, Local resolution map of the NCP-NSD3C complex final density map. e, FSC curve of the NCP-NSD3C complex final density map.