Skip to main content
. 2021 Feb 17;7:34. doi: 10.1038/s41420-021-00414-2

Fig. 2. Lithium rescues mushroom bodies morphology in CdLS D. melanogaster through WNT activation.

Fig. 2

A Schematic representation of the drug-treatment protocol. B–D Mushroom bodies of adult animals labeled with anti-FasII are shown in green. B Normal morphology of mushroom bodies was observed in yw controls where α, β, and γ lobes can be distinguished by their distinct projection patterns (arrows). C Abnormal mushroom bodies morphology was observed in Nipped-B407 haploinsufficient adults with a twisted structure (arrowhead in the upper brain) or lacking both α lobes (arrowhead in the lower brain) as example. D Rescue of mushroom bodies morphology is shown in Nipped-B407 haploinsufficient adults upon treatment with LiCl. E The table reports the total number and percentage of normal or abnormal adult mushroom bodies. Data were analyzed using Fisher’s exact test (***p < 0.005). F–I Histograms show arm (F, G) and en (H, I) gene expression levels in whole flies as 2-ΔΔCt ± SD. Analyses of yw controls are shown in the panels (F–H) and analyses of the Nipped-B407 mutants are shown in the panels (H, I). White: water; dark blue: LiCl 100 mM treatment. ***p < 0.005; * LiCl 100 mM vs H2O.