Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 17;7:34. doi: 10.1038/s41420-021-00414-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Analysis of the effects of PCI34051 and lithium exposure on the proliferation capabilities of the NSCs. White: NSCs treated with DMSO; yellow: NSCs treated with PCI34051; light blue: NSCs treated with DMSO + LiCl; dark green: NSCs treated with PCI34051 and LiCl. *** p < 0.001; $ p < 0.05; * DMSO vs PCI34051; $ PCI34051 vs PCI34051 + LiCl. B Analysis of the effects of Nipbl knockdown and lithium exposure on the proliferation capabilities of the NSCs. White: NSCs treated with AllStar; light green: NSCs treated with siRNA against Nipbl; light blue: NSCs treated with AllStar and LiCl; dark green: NSCs treated with siRNA against Nipbl and LiCl. *** and °°° p < 0.001; * AllStar vs siRNA against Nipbl; ° siRNA against Nipbl vs siRNA against Nipbl + LiCl. C Representative pictures of the neurospheres growing in the well during various treatments as in panel (A). Scale bar: 2 mm. D Representative pictures of the neurospheres growing in the well during various treatments as in panel (B). E Analysis of the effects of PCI34051 and lithium exposure on the gene expression of Ccnd1 in the NSCs. White: NSCs treated with DMSO; yellow: NSCs treated with PCI34051; light blue: NSCs treated with DMSO + LiCl; dark green: NSCs treated with PCI34051 and LiCl. ** p < 0.01; * p < 0.05; ** DMSO + LiCl vs PCI34051; * PCI34051 + LiCl vs PCI34051. F Analysis of the effects of PCI34051 and lithium on the differentiation capabilities of the NSCs. Cells were immunostained for neuron and nuclei detection. White: NSCs treated with DMSO; yellow: NSCs treated with PCI34051; light blue: NSCs treated with LiCl; dark green: NSCs treated with PCI34051 and LiCl. G Comparison of the effects of PCI34051 and PCI34051 + LiCl of NSCs differentiation. ** p < 0.01; * PCI34051 vs PCI34051 + LiCl. H Representative staining of the differentiated NSCs. Differentiated neurons were labeled with β-tubulin III antibody (red) and nuclei were labeled with DAPI (blue). Scale bar: 50 µm.