Fig. 2.

Clustering of baseline biopsies and matched rebiopsies from 29 EGFR wild type NSCLC patients. a Heatmap for the expression of 201 immune and cancer-related genes. Columns represent NSCLC biopsies and rows represent genes. Expression values have been centred and scaled for each row for better visualization. Rows and columns have been grouped using unsupervised hierarchal clustering. Two patient subgroups were derived from the clustering and are indicated above the heatmap: (violet) subgroup 1 (n = 38), (orange) subgroup 2 (n = 20). Blue and yellow bars indicate baseline biopsies and rebiopsies, respectively. b Frequency of paired pre- and post-therapy samples found to cluster together as neighbours or apart after treatment with CIS + PEM or CIS + PEM + BEV. Statistics: McNemar test was used to calculate the significance of matched samples to cluster apart from each other into subgroup 1 and subgroup 2. Chi-square test was used to calculate cluster as neighbours or apart from each other