Table 1.
Primer Sequences and Probe Number for Real-time PCR.
| Target human genes | Foward primer | Reverse primer | Probe number |
|---|---|---|---|
| POU5F1 | cttcgcaagccctcatttc | gagaaggcgaaatccgaag | 60 |
| LIN28A | ccgtgtccaaccagcagt | acgttgaaccacttacagatgc | 83 |
| HNF4A | cagcactcgaaggtcaagcta | acgggggaggtgatctgt | 66 |
| AFP | atggccatcaccagaaaaat | cataagtgtccgataataatgtcagc | 66 |
| ALB | gtgaggttgctcatcggttt | gagcaaaggcaatcaacacc | 7 |
| CYP3A4 | gatggctctcatcccagactt | agtccatgtgaatgggttcc | 2 |
| ACTN2 | catgatccaggaggaggaggagt | acaccaggcagtgaaggtct | 7 |
| NKX2.5 | cacctcaacagctccctgac | aatgcaaaatccaggggact | 7 |
| GAPDH | agccacatcgctcagacac | gcccaatacgaccaaatcc | 60 |