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The recognition substance of effector proteins of each subtype in type V family. Among them, Cas12a, Cas12b, Cas12c, Cas12d, Cas12h, Cas12i, and Cas12j were confirmed to have the ability to interact with both dsDNA and ssDNA. Cas14 only has the ability to act on ssDNA. Cas12g can recognize ssRNA. When the single-stranded nucleic acid molecule is used as a substance, the binding of Cas protein is not restricted by PAM.
