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. 2021 Feb 4;11:599111. doi: 10.3389/fpls.2020.599111

FIGURE 6.

FIGURE 6

Effect of DA-6 treatment on active oxygen accumulation in tomato leaves under low night temperature. (A) Histochemical staining of tomato leaves under various treatment conditions with tetranitroblue tetrazolium chloride (NBT). (B) Histochemical staining of tomato leaves under various treatment conditions with diaminobenzidine (DAB). (C) O2 generation rate in leaves. (D) H2O2 content in leaves. Data are the means of five replicates with standard errors shown by vertical bars. Differences among treatments were analyzed by the one-way ANOVA comparison test (P < 0.05). Different letters indicate significant differences among treatments. CK, the plant grown at optimal temperature (28°C/18°C); LHT, the plant grown at low night temperature (28°C/6°C); LHT + DA-6, the plant pretreated with DA-6 and grown at LHT.