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. 2021 Feb 3;26:100935. doi: 10.1016/j.bbrep.2021.100935

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Maintenance of oNK-1 culture is IL-2 dependent. (A) CD16⁺ population of oNK-1 cells at different concentrations of IL-2 was monitored by flow cytometry. (B) The expansion of oNK-1 cells at different concentrations of IL-2 was recorded. (C) Cell viability (filled square) and total cell number (filled circle) were determined by the trypan blue exclusion method. The viability and cell number of oNK-1 cells were monitored for 30 days. (D) The percentage of CD16-expressing oNK-1 cells was monitored by flow cytometry for more than 200 days.