Figure 1.

Small airway BC from HAART-treated HIV⁺ nonsmokers are induced to release inflammatory mediators in vivo. Small airway BC (5 × 10⁵ in 1 ml) from 4 HIV^- and 4 HIV⁺ nonsmokers (all with viral load < 20 copies/ml) were plated onto type IV collage-coated 6-wells and incubated for 2 days. Culture supernatants were collected for assessment of cytokine release. (A) Western analyses of IL-8, IL-1β, GM-CSF and ICAM-1 from HIV^- (lanes 1–4) and HIV⁺ nonsmokers (lanes 5–7). The original images of full-length immunoblot for each cytokine were shown in Supplemental Fig. 1. (B) Quantification of IL-8, IL-1β, GM-CSF and ICAM-1 release by small airway BC. Western analysis from HIV‾ and HIV⁺ small airway BC (n = 4 for each group) were quantified by ImageJ software and normalized with the total amount of supernatant protein as densitometry (pixel intensity) per µg of supernatant protein.