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. 2021 Feb 17;12:1086. doi: 10.1038/s41467-021-21423-w

Fig. 1. Photoinduced dynamics of ferrous cytochrome c observed with femtosecond X-ray emission and scattering.

Fig. 1

a Heme environment of reduced horse heart cytochrome c (cyt c) with Met80 and His18 axial ligands. b Ferrous heme ground state electronic configuration of cyt c. The dashed arrow indicates the photoexcitation process. c Scheme of the experimental setup (adapted from Kjaer et al.20—published by The Royal Society of Chemistry). The X-ray pulses probe laser-induced changes from the cyt c liquid jet sample. 2D images of the Fe Kβ X-ray emission spectra and the X-ray scattering in the forward direction are simultaneously read out shot-by-shot. The Fe Kβ X-ray emission signal is collected using a high-energy resolution X-ray emission spectrometer based on the von Hamos geometry.