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. 2021 Feb 17;11:4019. doi: 10.1038/s41598-021-83249-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Amygdala segmentation and regions of interest. (A) Segmentations of the right (blue) and left (red) amygdala. Segmentations of each participant’s brain were obtained from raw T1 images with Multi-atlas Cortical Reconstruction Using Implicit Surface Evolution (MaCRUISE) software and were used to analyze the relationship between maternal anxiety and child amygdala volume. (B) Amygdala seed regions used in the functional connectivity analysis. Regions are overlayed on a T1 image registered to a pediatric brain template in standard space. Seed regions were defined with a 6 mm radius sphere centered at − 25, 1, − 18 (red; left amygdala) and 26, 1, − 18 (blue; right amygdala). Coordinates were obtained from the Automated Anatomical Labeling atlas (AAL). S—superior; I—inferior; R—right; L—left. The segmentations and regions of interest are displayed on the same participant.