Fig. 2. WD_McB feeding stimulates induction of gut-specific regulatory T cells.

A–D Number of indicated cells in colon. E–G Representative plots of colonic TCRβ⁺CD4⁺ FoxP3⁺ RORγt⁺ _pT_regs (left) and IL-17⁺ _pT_regs (right) in LFD (E), WD_CNTL (F), and WD_McB (G) group. H–K Number of indicated cells in small intestine. L–N TCRβ⁺CD4⁺ FoxP3⁺ RORγt⁺ _pT_regs (left) and IL-17⁺ _pT_regs (right) in LFD (L), WD_CNTL (M), and WD_McB (N) group. O–R Percentage of indicated cells in SI- and LI-LP from ‘weight-matched’ mice housed at thermoneutrality. A–N Dots indicate individuals data points in Exp1 (squares) and Exp2 (circles) with n = 6 (LFD) or 10 (WD_McB and WD_CNTL). O–R SI-LP n = 5 per group and LI-LP n = 9 (LFD and WD_REF groups) or 11 (WD_McB). A–D, H–K, O–R Bars indicate group mean ± SEM and dots indicate individual data points. All p-values < 1 × 10⁻¹ between WD_CNTL and indicated group by one-way ANOVA with multiple comparisons and Dunnett post-hoc are depicted.