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. 2021 Feb 17;12:1097. doi: 10.1038/s41467-021-21341-x

Fig. 2. A specific copy of mTALT is duplicated in a constant orientation.

Fig. 2

a (Left) Alignment of the mate reads of telomere-containing reads. (Right) Alignment of the mate reads of telomere-containing reads in a strand-specific way. b (Top) Schematics of the tandemly duplicated mTALTs and the specific primers used. (Bottom) PCR assays showing the directionality of mTALT duplication. c Calculated copy numbers of mTALTs from WGS data of various mouse strains. d PCR assays elucidating the presence of mTALT on chromosome 11. e Schematics of the mTALT region in chromosomes 11 and 13 of the reference (C57BL/6J) and 129/Ola genome. f SNP analysis of the mTALT region at each time point. The connected lines denote groups sharing SNPs. g Number of SNPs sorted by allelic zygosity. h Frequencies of representative SNPs in mTALT region. i Calculated copy numbers of mTALTs from independent ALT survivors. The bars represent the means and SDs from three biologically independent replicates. Source data are provided as a Source Data file.