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. 2021 Feb 17;11:4030. doi: 10.1038/s41598-021-83612-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pembrolizumab increases IFNγ and IL-10 levels in cultures of PBMCs stimulated with EBV peptides. Ability of pembrolizumab (anti-PD-1; 1.0 μg/ml) to modify IFNγ (A) or IL-10 (B) levels in cultures of PBMCs stimulated with EBV peptides. (A,B) indicate responses of individual donors (each colour represents an individual donor). (C) indicates percentage change in response in the presence of pembrolizumab relative to isotype control (mean + SEM, n = 12). The impact of pembrolizumab was statistically significant compared to isotype control (*one sample T-test).