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. 2021 Feb 4;8:608544. doi: 10.3389/fcell.2020.608544

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Copyright © 2021 Li, Li, Li, Deng, Hu and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of Wnt10b and PTEN on BMP9-induced osteogenesis in C3H10T1/2 cells. (A) 3-D reconstruction of μ-CT scanning results shows the effect of silencing Wnt10b and/or PTEN on BMP9-induced bone formation in C3H10T1/2 cells (representative data are shown). (B) Quantitative analysis μ-CT scanning results show the effect of silencing Wnt10b and/or silencing PTEN on BMP9-induced bone formation in C3H10T1/2 cells (TV, total volume; BV, bone volume; BS, bone surface area; Tb.N, trabecular number; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation). (C) H and E staining results show the effect of silencing Wnt10b and/or PTEN on BMP9-induced bone formation in C3H10T1/2 cells (Scale is 200 μm for upper panel; scale bar is 50 μm for lower panel).