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The primers used for PCR.
| Gene | Primer | Sequence (5′ → 3′) |
|---|---|---|
| Wnt10b | F | GGATGGAAGGGTAGTGGTGA |
| R | CTCTCCGAAGTCCATGTCGT | |
| Runx2 | F | GCCGGGAATGATGAGAACTA |
| R | GGACCGTCCACTGTCACTTT | |
| PTEN | F | CATAACGATGGCTGTGGTTG |
| R | CGGGGTAAGGCTGTTTTACA | |
| GAPDH | F | ACCCAGAAGACTGTGGATGG |
| R | CACATTGGGGGTAGGAACAC | |
| Wnt10b (ChIP) |
Primer1 F | AGCTAGGAGGGTGAGTCAGG |
| Primer1 R | TGCTGCACAAGAGATGAGGG | |
| Primer2 F | GCTGGCCCATCTCAGAAGTT | |
| Primer2 R | GCTTCCTTGATGAGGGTGCT | |
| Primer3 F | GTCCTCAGCGTGTCAAAGGA | |
| Primer3 R | GAGTTCCACTCACCTGCTCC |
F, forward; R, reverse.
