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Scheme of sample preparation for massive analysis of cDNA ends (MACE) analysis. The uninfected larvae, originating from three individual females fed on a single guinea pig, were mixed and fed on B. afzelii-infected or uninfected mice. The nymphs then were fed on uninfected mice and dissected for midguts (150–220 nymphs for each group) at the three indicated time points. The MACE analyses were performed on six different RNA pools (MACE 1-6).
