Figure 2.

Expression of tick midgut genes is altered in the presence of Borrelia afzelii. Volcano plots showing differentially expressed tick transcripts analyzed by MACE at individual time points. (A) Unfed nymphs (B) Nymphs fed for 24 h (C) Fully fed nymphs. n = number of differentially expressed transcripts. Total differentially expressed transcripts (black), upregulated transcripts (red; p-value ≤ 0.05 and log2 fold change ≥ 2.3), and downregulated transcripts (blue; p-value ≤ 0.05 and log2 fold change ≤ −2.3). up = total upregulated transcripts, down = total downregulated transcripts, MG, midgut.