Table 2.
Example of plate layout for trypsin optimization
| [HDAC] [Trypsin] |
1 | 2 | 3 | 4 |
|---|---|---|---|---|
| A | NE 200 μg/mL |
NE 100 μg/mL |
NE 50 μg/mL |
NE 25 μg/mL |
| B | NE 200 μg/mL |
NE 100 μg/mL |
NE 50 μg/mL |
NE 25 μg/mL |
| C | 100 nM 200 μg/mL |
100 nM 100 μg/mL |
100 nM 50 μg/mL |
100 nM 25 μg/mL |
| D | 100 nM 200 μg/mL |
100 nM 100 μg/mL |
100 nM 50 μg/mL |
100 nM 25 μg/mL |
| E | 10 nM 200 μg/mL |
10 nM 100 μg/mL |
10 nM 50 μg/mL |
10 nM 25 μg/mL |
| F | 10 nM 200 μg/mL |
10 nM 100 μg/mL |
10 nM 50 μg/mL |
10 nM 25 μg/mL |
| G | 1 nM 200 μg/mL |
1 nM 100 μg/mL |
1 nM 50 μg/mL |
1 nM 25 μg/mL |
| H | 1 nM 200 μg/mL |
1 nM 100 μg/mL |
1 nM 50 μg/mL |
1 nM 25 μg/mL |
NE, no enzyme added.