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. 2021 Feb 3;2(1):100313. doi: 10.1016/j.xpro.2021.100313

HEPES assay buffer – solution B

Reagent Final concentration Amount
HEPES sodium salt 50 mM 7.808 g
KCl 100 mM 2.984 g
TCEP HCl salt 0.2 mM 23 mg
Tween 20 0.001% 4.0 μL
ddH2O n/a 400 mL
Total n/a 400 mL