Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 17;11:3991. doi: 10.1038/s41598-021-83603-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Suppression of NF-κB reduces HIF-1α expression and glucose metabolism in MYD88 L265P-expressing p53^−/−MEFs. (a–d) Suppression of NF-κB using IκB SR in p53^−/−MEFs expressing MYD88 L265P. (a) Total cell lysates were analysed by immunoblotting. The arrow indicates phosphorylated-IκBα and the asterisk indicates a nonspecific band. (b) Gene expression of NF-κB targets measured by qPCR. (c) Expression of glucose metabolism-related genes were quantified by qPCR. (d) Glucose uptake and lactate production were measured. The quantified results are presented as the mean ± s.d. (n = 4) using one-way ANOVA followed by Scheffe’s F test. *P < 0.05, **P < 0.01. (e,f) p53^−/−MEFs were treated with LPS and IL-1β for the indicated times. (g) Endogenous MYD88 expression were reduced by shRNA (#1 and #2), then these cells were stimulated with LPS for the indicated times. (b,c) The y-axis values are relative fold change for gene transcripts normalised to β-actin. Data represent the mean ± s.d. (n = 3) using one-way ANOVA followed by Scheffe’s F test. *P < 0.05, **P < 0.01.