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. 2021 Feb 17;11:3991. doi: 10.1038/s41598-021-83603-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Suppression of HIF-1α does not affect NF-κB activation but reduces glucose metabolism in MYD88 L265P-expressing p53^-/-MEFs. Reduced HIF-1α expression by shRNA (#1 and #2) in p53^−/−MEFs expressing MYD88 L265P. (a) Total cell lysates were analysed by immunoblotting. (b) Gene expression of NF-κB targets measured by qPCR. (c) Expression of glucose metabolism-related gene mRNAs quantified by qPCR. (d) Glucose uptake and lactate production were measured using shHif1a #1. The quantified results are presented as the mean ± s.d. (n = 4) using one-way ANOVA followed by Scheffe’s F test. *P < 0.05, **P < 0.01. (b,c) The y-axis values are the relative fold change for gene transcripts normalised to β-actin. Data represent the mean ± s.d. (n = 3) using one-way ANOVA followed by Scheffe’s F test. *P < 0.05, **P < 0.01.