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. 2021 Feb 17;11:3955. doi: 10.1038/s41598-021-83115-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Frequency tuning is not altered by early malleus displacement. (A) Forward masked tuning curves were obtained with the probe at 20 dB above threshold, and are depicted normalized to sensation level to allow comparison across treatment groups. Normalized tuning curves were fit using a roex filter function. Masked thresholds are plotted for 1/10 octave frequency steps of maskers surrounding each center probe frequency at 1, 2, 4, 8 and 16 kHz. (B) Q₁₀ values derived from roex filter functions indicate that conductive hearing loss did not significantly broaden tuning at 10 dB above threshold. (C) There are no significant correlations between CAP amplitude and Q10 values for either group at any probe frequency, indicating that synaptopathy does not correlate with cochlear tuning. Error bars are SEM.