Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 17;4:223. doi: 10.1038/s42003-021-01715-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — A Phylogenetic tree based on full-sequence alignment consisting of 12 TPS homologs from A. aegerita (Aa.LNS and Agr), 11 from A. pediades (Ap.LS, Ap.LNS and Agrped1), 3 from Galerina marginata (Galma), 2 from Hypholoma sublateritium (Hypsu1), and 1 from Hebeloma cylindrosporum (M413_27416). B Sequence similarity network (SSN) built on the predicted active sites (Supplementary Table S1). Agr10 is excluded as it shares limited similarity to the templates (PDB ID: 4LXM and 5NX5). Instead, D5SL78 from Streptomyces clavuligerus and D8RNZ9 from Selaginella moellendorffii (Spikemoss) are included. The rest of the candidates are the same as those of full-sequence alignment. LNS and muurolene/cadinene synthase groups are highlighted in green and blue, respectively. C Experimental validation of the predicted fungal TPS homologs. Ap.LS produced exclusively linalool; the other four produced both nerolidol (85–98%) and linalool (15–2%). Here percentage (%) refers to the peak area ratios of linalool or nerolidol to the sum of the peak areas of both substances present in each chromatogram.