Fig. 4. In vitro characterization of Aa.LNS and Ap.LS and in vivo comparison of linalool synthases from different kingdoms in linalool bioproduction.

A SDS-PAGE gel image of purified Aa.LNS and its kinetic analysis. B Protein gel of purified Ap.LS and its kinetic analysis (no product was observed for FPP). C In vivo linalool yield comparison of linalool synthases from a fungus, a bacterium, and a plant (error bars, mean ± s.d., n = 3). D The OD₆₀₀ of different strains in Fig. 3C. Ap.LS, Sc.LNS, and Cb.LS from Clarkia breweri (Q96376) were selected as the representative of kingdoms fungi, bacteria, and plantae, respectively.