Figure 2.

Efficacy of Piezo1 knockdown in human trabecular meshwork cells. (A) Relative Piezo1 mRNA levels in the human trabecular meshwork treated with control or Piezo1 siRNAs 24 h after transfection, as determined by qPCR. Data are normalized to GAPDH mRNA level. Data are presented as means ± SE (n = 4 samples of cDNA synthesized from RNA extracted from cells in 4 wells). **p < 0.01, Dunnett’s test. (B) Representative images illustrating intracellular Ca²⁺ changes in human trabecular meshwork cells transfected with control or Piezo1 siRNAs and stimulated with Piezo1 agonist Yoda1. Scale bar, 100 μm. (C) Time course of Yoda1-induced Ca²⁺ changes in control or Piezo1 siRNA-transfected human trabecular meshwork cells. (D) Yoda1-evoked intracellular Ca²⁺ elevation in human trabecular meshwork cells transfected with control or Piezo1 siRNAs. Data are expressed as the means ± SE (n = 4 experiments; ≥ 20 cells/experiment). **p < 0.01, Dunnett’s test.