Table 1.
Primers specific for TRPV1-6, PIEZO1-2, GAPDH, and MYOC.
| Gene Name | Primer | Sequences (5′–3′) | Product Size(bp) |
|---|---|---|---|
| TRPV1 | Forward | AAGTTCCTGCTGCAGAACTC | 130 |
| Reverse | TGCTCGTCACAAACTTCGTG | ||
| TRPV2 | Forward | AAACTGCTGCAGGCGAAATG | 95 |
| Reverse | GCAACAGCGGTGAAGATGAAC | ||
| TRPV3 | Forward | GTTAGCTACCCGCATTAAGCCTGA | 107 |
| Reverse | AGCAATTCTGGAATTCCCAGCTC | ||
| TRPV4 | Forward | TGCATGCGCCACCATTTTTG | 114 |
| Reverse | TATTGAGCACCGGCAAATCC | ||
| TRPV5 | Forward | GGAGACCTAATGCGTTTCTGCTG | 197 |
| Reverse | AAGGGCAAGTCCACGTCGTA | ||
| TRPV6 | Forward | AGCAGTGCCAATTGGGAAAG | 121 |
| Reverse | TGAGAACACGCAGTCAGATCTG | ||
| PIEZO1 | Forward | ATGCCAACGAGAAGCACATG | 128 |
| Reverse | ACGGATGTACTTGGGGAAGAG | ||
| PIEZO2 | Forward | ATTTCATTGTGCGGCCCAAC | 127 |
| Reverse | TTGTCACCTGCCATGATTCG | ||
| GAPDH | Forward | AATTCCATGGCACCGTCAAG | 104 |
| Reverse | ATCGCCCCACTTGATTTTGG | ||
| MYOC | Forward | TACACGGACATTGACTTGGC | 159 |
| Reverse | ATTGGCGACTGACTGCTTAC |