TABLE 1.
Detection of inactivated HAV in water (molecular biology grade) over time at −80°C, −20°C, 4°C, and 23°C starting from an initial load of 2.5 × 102 in 100 μL.
| Temperature (°C) | −80 | −20 | 4 | 23 | |
| Time | 30 min | 3 | 3 | 3 | 3 |
| 6 h | 3 | 2 | 2 | 2 | |
| 1 day | 2 | 2 | 3 | 3 | |
| 2 days | 2 | 3 | 3 | 0 | |
| 4 days | 2 | 2 | 2 | 2 | |
| 8 days | 2 | 3 | 3 | 2 | |
| 16 days | 2 | 3 | 1 | 2 | |
| 60 days | 2 | 3 | 0 | 0 | |
| 90 days | 2 | 1 | 2 | 0 | |
Values are the number of positive RT-qPCR results out of three tests for each experimental condition. Bold was used to present the conditions where RNA was not detected.