TABLE 2.

Detection of inactivated HAV in PBS stored at −80°C, −20°C, 4°C, and 23°C.

Temperature (°C)		−80			−20			4			23
Genome copies/sample		250	25,000	2.5E6	250	25,000	2.5E6	250	25,000	2.5E6	250	25,000	2.5E6
Time	0.5 h	1	2	2	2	2	2	2	2	2	0	2	2
	6 h	0	1	2	1	1	1	0	2	2	1	1	2
	1 day	2	2	1	1	2	2	2	2	2	0	1	2
	2 days	0	2	2	0	1	2	0	1	2	0	2	2
	4 days	0	2	2	1	2	2	0	1	2	1	2	2
	8 days	1	2	1	0	1	2	0	0	2	0	2	2
	16 days	1	1	1	1	1	1	0	1	1	0	1	1
	60 days	1	1	2	0	2	2	0	1	2	0	0	1
	90 days	1	2	2	0	1	1	0	2	1	0	1	2

Values are the number of positive RT-qPCR results out of two tests for each experimental condition. Sample volume was 100 μL. Bold was used to present the conditions where RNA was not detected.