FIGURE 2.

Treatment of MHY1485 can effectively promote human follicle growth and restore endocrine functions. Human ovarian fragments were cultured with 10 μM MHY1485-added media (activated group) or culture media only (control group) in vitro. Histological analyses showed that there was no obvious necrosis or morphological change when the two groups were compared. (A) Control group treated without MYH1485, Bars: 100 μm. Activated group treated with MYH1485, Bars: 100 μm. (B) No abnormal histological affected on human ovarian tissues after transplantation. (C) Graft weight changed. After treatment of MHY1485 one month, grafts weight increased compared with the control group. Data was applied paired t test for statistical analysis and presented as the mean ± SEM **P < 0.01, N = 6. (D) Following histological analyses, the development of larger growing follicles was observed in the MYH1485-treated ovarian tissues, Bars: 50 μm. Whereas primary follicles remained dormant in the untreated ovarian tissues, Bars: 50 μm. (E) Follicle counting results (right: total follicle numbers per fragment; left: follicle dynamics), showed that the number of growing follicles increased after activation with 1 month. Data was applied paired t test for statistical analysis and presented as the mean ± SEM *P < 0.05, N = 6. (F) FSH (ng/ml) elevated after the surgical castration of the SCID mice and decreased 1 month after activated grafts were transplanted into host mice, **P < 0.01, N = 6. AMH (ng/ml) dropped after surgical castration and slightly increased 1 month after activation (normal:untreatment; castrated:after castrated without other treatment; control:castrated with normal medium treatment; MHY1485: castrated with MHY1485 medium treatment). Data was applied ANOVA for statistical analysis and presented as the mean ± SEM **P < 0.01, ****P < 0.0001, N = 6.