Fig. 1.

Flowchart of the experimental setup used to collect parasite sacs from infected bryozoa for sequencing. (A) Laboratory infected bryozoan Fredericella sultana colony. (B) Infected zooid containing the parasite sacs (arrows). F. sultana zooids from different colonies were used for the collection of parasite sacs. (C) Isolated pure parasite sacs. RNA was isolated from 6 parasite sacs samples. In a total ∼111.5 million clean paired-end reads were obtained. De novo assembly of filtered reads was performed with CLC Genomic Workbench 12 software and was annotated using Blast2GO.