Figure 3.

Daunorubicin (Daun) and etoposide (Etop) treatment perturbed SETD7 recruitment and H3K4me1 level on MyHC‐IId gene. (A) Western blot showing indicated changes in protein expression status upon Daun and Etop treatment. (B) Chromatin immunoprecipitation (ChIP) assays were performed with the specified antibodies. Coloured boxes in the inset represent primer pairs of the corresponding gene promoter used to amplify ChIPed DNA template by quantitative polymerase chain reaction (qPCR). Individual bars show the status of SETD7 chromatin occupancy on indicated genes after Daun treatment. Data represented as an average of at least three independent experiments with triplicates used in each experiment. (C) Same as in (B), except anti‐rabbit H3K4me1 antibody was used for ChIP to measure H3K4me1 marks on the indicated gene after Daun treatment. (D) Same as in (C), except ChIP assays were performed with Etop‐treated myotubes.