Skip to main content
. 2020 Dec 10;12(1):159–176. doi: 10.1002/jcsm.12645

Figure 5.

Figure 5

Chemotherapeutic drugs impede SETD7–p300 association and p300 chromatin targeting. (A) Western blot shows p300 and other indicated protein level after daunorubicin (Daun) and etoposide (Etop) treatment. (B) Upper panel shows SETD7–p300 interaction. The endogenous SETD7 was immunoprecipitated (anti‐mouse SETD7 antibody) from control vs. Daun‐treated myotubes and probed with p300 antibody. The lower panel shows the input control probed with indicated antibodies. (C) Chromatin immunoprecipitation (ChIP) assays were performed from Daun‐treated myotubes (nearly 10 million) extract using p300 antibody. Coloured boxes represent primer pairs for corresponding genes. (D) Same as in (C), except ChIP assays were performed from Etop‐treated and roughly 2 million myotubes. (E) Similar to (C), except ChIP assays were performed from with anti‐RNA pol II (phosphorylated at ser5) antibody with chromatin originated from Daun and Etop‐treated myotubes, respectively. (F) Western blot showing RNA pol II level after Daun and Etop treatment. qPCR, quantitative polymerase chain reaction.