Fig. 5.

IPTG titration to modulate the expression of dxs and idi in the MEP pathway in Synechococcus 2973 limonene-producing strains. (A) Limonene biosynthetic pathway. The overexpressed enzymes were highlighted in blue color. (B) Limonene production of strains with IPTG titration. The experiment was conducted in 1% CO₂ and 250 ​μmol photons m⁻² ​s⁻¹ light for 2 days. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)