Table 1.
Plasmids and strains used in this study.
| Plasmids | Description | Reference |
|---|---|---|
| pRL443 | Conjugal plasmid for bacterial conjugation | Elhai et al. (1997) |
| pRL623 | Helper plasmid for bacterial conjugation | Elhai et al. (1997) |
| pSL3383 | CRISPR/Cpf1 plasmid which generates SomB Y380H mutant | This study |
| pSL3384 | CRISPR/Cpf1 plasmid which generates CrtE R299Q mutant | This study |
| pSL3385 | Ptrc1O-lims-RBS (TIR=4743)-gpps, RSF1010 plasmida | This study |
| pSL3386 | Ptrc1O-lims-RBS (TIR=1008)-gpps, RSF1010 plasmida | This study |
| pSL3387 | Ptrc1O-lims-RBS (TIR=3146)-gpps, RSF1010 plasmida | This study |
| pSL3388 | Ptrc1O-lims-RBS (TIR=5635)-gpps, RSF1010 plasmida | This study |
| pSL3389 | Ptrc1O-lims-RBS (TIR=8185)-gpps, RSF1010 plasmida | This study |
| pSL3390 | Derived from pSL3385, gpps-eyfp fusion construct | This study |
| pSL3391 | Derived from pSL3386, gpps-eyfp fusion construct | This study |
| pSL3392 | Derived from pSL3387, gpps-eyfp fusion construct | This study |
| pSL3393 | Derived from pSL3388, gpps-eyfp fusion construct | This study |
| pSL3394 | Derived from pSL3389, gpps-eyfp fusion construct | This study |
| pSL3395 | pSL3385 carrying PlacUV5-dxs | This study |
| pSL3396 | pSL3385 carrying PlacUV5-idi | This study |
| pSL3397 | pSL3385 carrying PlacUV5-dxs-idi | This study |
| Strains | Description | Reference |
| WT | Synechococcus 2973 wild type | Yu et al. (2015) |
| High Lim | Synechococcus 2973 somB Y380H crtE R299Q. High limonene-producing strain | This study |
| Low Lim | Synechococcus 2973 somB L234S. Low limonene-producing strain | This study |
a
TIR, translation initiation rate.