Fig. 3.

Kinetics of PCSK9_62 conversion to PCSK9_55 in vitro. Pulse-chase experiments traced the rate of PCSK9 production intracellularly and the rate of secretion to the extracellular space. Storage phosphor screen scans show PCSK9 presence over time (0–22 h) in cell extracts (A) and media (B) of HEK293T cells cotransfected with pPCSK9 62 and empty vector (empty circles) or pPCSK9 62 and pFURIN (full circles). Quantification of PCSK9_62 bands from “A” are plotted relative to pPCSK9 62 and empty vector condition at time 0 h, whereas for “B”, the 7 kDa peptide bands are plotted relative to pPCSK9 62 condition and empty vector at time 1 h. Image is a representative of 3 independent experiments. Values are mean ± SEM. Cells transfected with pcDNA3 were used as a negative control, and pPCSK9 55 was used as a reference to identify intracellular PCSK9_55.