TABLE 3.
Antimicrobial susceptibility of S. aureus ATCC 29213 mutants isolated by serial passage for 100 days.
| Mutant strain | Mutated gene | MIC (μg/ml) | ||||
| IBG | VAN | LNZ | CIP | GEN | ||
| SP1-1 | 4 | 1 | 1 | 0.25 | 0.5 | |
| SP1-58 | pnp_1, atpE, walR, pcrA, ybiV, Org1_01939, Org1_01695, Org1_01489 | 8 | 2 | 0.5 | 0.25 | 0.5 |
| SP1-99 | rsmG, atpE, dtd3,walR, pcrA, Org1_01695, Org1_01489, prkC, lip2, mnhG1 | 8 | 2 | 0.5 | 0.5 | 0.5 |
| SP2-1 | 4 | 1 | 1 | 0.25 | 0.5 | |
| SP2-58 | rpoE, atpE, walR, pcrA, ybiV, Org1_01695, Org1_01701, Org1_01489, Org1_01939, Org1_00143, pnp_1 | 16 | 2 | 0.5 | 0.5 | 0.5 |
| SP2-100 | nusG, atpE, pcrA, isdG_2, Org1_00312 | 8 | 2 | 1 | 0.25 | 0.5 |
| SP3-1 | 4 | 1 | 1 | 0.25 | 0.5 | |
| SP3-34 | atpE, walR, pcrA, ybiV, Org1_01489, lip2 | 8 | 2 | 1 | 0.5 | 0.5 |
| SP3-66 | Org1_00874, atpE, Org1_00657, walR, pcrA, ybiV, Org1_01695, Org1_01489, Org1_01939, Org1_00143, pnp_1 | 8 | 2 | 0.5 | 0.25 | 0.5 |
| SP3-100 | Org1_00874, atpE, Org1_00657, walR, pcrA, ybiV, sasA, Org1_01939, Org1_00143, tcaR, pnp_1, glpK | 8 | 2 | 0.5 | 0.25 | 0.5 |
MIC, minimum inhibitory concentration; IBG, isopropoxy benzene guanidine; VAN, vancomycin; LNZ, linezolid; CIP, ciprofloxacin; GEN, gentamicin. SP1, SP2, and SP3 indicate three independent S. aureus 29213 cultures. Kb, S. aureus 29213 cells treated with 0.1% DMSO.