Skip to main content
. 2021 Feb 4;12:620915. doi: 10.3389/fmicb.2021.620915

TABLE 1.

Culture conditions and properties of Cyanothece 51142 during N2 and NO3 growth.

Culture Collection time N2 NO3 SC-N2
Culturing strategy Turbidostat Turbidostat Semi-continuous batch
Specific growth rate (d–1)a Daily average 0.22 ± 0.07 0.31 ± 0.05 0.26 ± 0.09
Cell diameter (μm) Dawn 2.92 ± 0.07 3.03 ± 0.09 2.99 ± 0.13
Chl a (fg cell–1) Dawn 109 ± 14 144 ± 9 111 ± 9
Cell C (fg cell–1) Dawn 2375 ± 23 2515 ± 159 3076 ± 127
Cell C (fmol cell–1) Dawn 198 ± 23 209 ± 13 256 ± 13
Cell N (fg cell–1) Dawn 534 ± 69 584 ± 30 550 ± 1.4
Cell N (fmol cell–1) Dawn 38 ± 5 42 ± 2 39 ± 1
C:N (w:w) Dawn 4.45 ± 0.58 4.30 ± 0.35 5.60 ± 0.22
C:N (mol:mol) Dawn 5.19 ± 0.08 5.02 ± 0.08 6.53 ± 0.26
Polysaccharide content (fg glucose eq. cell–1) Dawn 1657 ± 702 1978 ± 976 1166 ± 454b
Polysaccharides (fmol C cell–1) 55 ± 23 66 ± 33 39 ± 15b
Cyanophycin content (fg arginine eq. cell–1) Light phasec 37 ± 22 81 ± 32 n.d.
Cyanophycin (fmol C cell–1) 2.1 ± 1.2 4.6 ± 1.8
Cyanophycin (fmol N cell–1) 1.1 ± 0.6 2.3 ± 0.9

Shown are mean ± SD values for three replicate cultures.a Determined by exponential fits of the OD720 signal from the on-board sensor in the turbidostats (Zavřel et al., 2015a) or by total volume displaced during daily dilution of semi-continuous batch culture.b Measured at 16:00.c Mean and SD calculated from three values measured 2, 7, and 14 h after the start of the light phase.