TABLE 1.
Culture conditions and properties of Cyanothece 51142 during N2 and NO3 growth.
Culture | Collection time | N2 | NO3 | SC-N2 |
Culturing strategy | Turbidostat | Turbidostat | Semi-continuous batch | |
Specific growth rate (d–1)a | Daily average | 0.22 ± 0.07 | 0.31 ± 0.05 | 0.26 ± 0.09 |
Cell diameter (μm) | Dawn | 2.92 ± 0.07 | 3.03 ± 0.09 | 2.99 ± 0.13 |
Chl a (fg cell–1) | Dawn | 109 ± 14 | 144 ± 9 | 111 ± 9 |
Cell C (fg cell–1) | Dawn | 2375 ± 23 | 2515 ± 159 | 3076 ± 127 |
Cell C (fmol cell–1) | Dawn | 198 ± 23 | 209 ± 13 | 256 ± 13 |
Cell N (fg cell–1) | Dawn | 534 ± 69 | 584 ± 30 | 550 ± 1.4 |
Cell N (fmol cell–1) | Dawn | 38 ± 5 | 42 ± 2 | 39 ± 1 |
C:N (w:w) | Dawn | 4.45 ± 0.58 | 4.30 ± 0.35 | 5.60 ± 0.22 |
C:N (mol:mol) | Dawn | 5.19 ± 0.08 | 5.02 ± 0.08 | 6.53 ± 0.26 |
Polysaccharide content (fg glucose eq. cell–1) | Dawn | 1657 ± 702 | 1978 ± 976 | 1166 ± 454b |
Polysaccharides (fmol C cell–1) | 55 ± 23 | 66 ± 33 | 39 ± 15b | |
Cyanophycin content (fg arginine eq. cell–1) | Light phasec | 37 ± 22 | 81 ± 32 | n.d. |
Cyanophycin (fmol C cell–1) | 2.1 ± 1.2 | 4.6 ± 1.8 | – | |
Cyanophycin (fmol N cell–1) | 1.1 ± 0.6 | 2.3 ± 0.9 | – |
Shown are mean ± SD values for three replicate cultures.a Determined by exponential fits of the OD720 signal from the on-board sensor in the turbidostats (Zavřel et al., 2015a) or by total volume displaced during daily dilution of semi-continuous batch culture.b Measured at 16:00.c Mean and SD calculated from three values measured 2, 7, and 14 h after the start of the light phase.