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. 2021 Feb 4;9:592946. doi: 10.3389/fcell.2021.592946

Figure 2.

Figure 2

TRPM8 channel expression in hBM-MSCs. (A) RT-PCR from total hBM-MSCs RNA. cDNA fragment corresponding to TRPM8 transcript (144 bp) in hBM-MSCs is shown. As controls we used HEK cells transfected with TRPM8 and non-transfected cells, respectively. RPL 27 (Name of the Ribosomal proteinL27, 120 bp) is the housekeeping gene (n = 3). (B) Western blot detected TRPM8 protein in hBM-MSCs and cells transfected with TRPM8 (130 kDa). β-actin (45kDA) was used as a load control. As a negative control we used untransfected cells. (C) Percentage of TRPM8 total and membrane expression in hBM-MSCs and control cells transfection (n = 3). (D) TRPM8 expression pattern in permeabilized hBM-MSCs (left panel) and control of unspecific secondary antibody binding (right panel) (n = 4). The images presented in the figure shown confocal images of Alexa fluor 488 (TRPM8, green) and DAPI (blue).