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. 2021 Feb 4;9:592946. doi: 10.3389/fcell.2021.592946

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Copyright © 2021 Henao, Grismaldo, Barreto, Rodríguez-Pardo, Mejía-Cruz, Leal-Garcia, Pérez-Núñez, Rojas, Latorre, Carvacho and Torres.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRPM8 hBM-MSC localization. Maximum image volume projection of hBM-MSCs after double staining with the antibody TRPM8 conjugated with Alexa 488 and Mitotracker Red FM (colocalization index 0.03) (Overlap 0.36) (A), Lisotracker Red (colocalization index 0.02) (overlap 0.49) (B), and Alexa 647 calnexin (colocalization index 0.14) (Overlap 0.60) (C). (D) Selected area for the colocalization analysis (perinuclear region). (E) Colocalization analysis (n = 3) experiments.