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. 2021 Feb 4;9:592946. doi: 10.3389/fcell.2021.592946

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Copyright © 2021 Henao, Grismaldo, Barreto, Rodríguez-Pardo, Mejía-Cruz, Leal-Garcia, Pérez-Núñez, Rojas, Latorre, Carvacho and Torres.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hBM-MSCs express functional TRPM8 channels. (A,B) Currents evoked in response to a voltage pulse protocol. Current- voltage (IV) curves in whole cell configuration in hBM-MSCs cells before (control) and after the addition of menthol 500 μM and BCTC 10 μM (n: 4). (Insert: Voltage step protocol). TRPM8 currents were calculated by subtracting the residual current after the addition of BCTC current from the current activated by menthol. (B) IV in response to a voltage ramp protocol in configuration of whole cell before (control, continues line) and after the addition of menthol 500 μM (dashed line) and BCTC 10 μM (dotted line) (n = 4). The ramp protocol used for the acquisition of the records is indicated in the insert. (C) Current density (pA/pF) from hBM-MSC at −80 mV and +80 mV, before and after menthol addition. *P < 0.05. Data are mean ± SD (n = 3).