REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
cOmplete, Mini, EDTA-free tablets	Roche	Cat#:11836170001
Formaldehyde 37%	Sigma-Aldrich	Cat#:F8775
DAPI	Sigma- Aldrich	Cat#:D9542
Glycogen	Thermo Fisher Scientific	Cat#:R0561
KAPA SYBR FAST for LightCycler480 qPCR Kit	Roche	Cat#:SFLCKB
Propidium iodide	Sigma-Aldrich	Cat#:P4170
Proteinase K	Roche	Cat#:RPROTK-RO
RiboLock RNase inhibitor	Thermo Fisher Scientific	Cat#:EO0381
Spermine 97%	Sigma-Aldrich	Cat#:S3256
Tris-HCl (1 M, pH 7.5)	Thermo Fisher Scientific	Cat#:15567027
Critical commercial assays
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase	Thermo Fisher Scientific	Cat#:K1671
Deposited data
Processed data from Col-0 nuclear and total fractions	(de Leone et al., 2020)	GEO:GSE120757
Experimental models: organisms/strains
Arabidopsis Col_ pSHR:NTF	This paper	N/A
Oligonucleotides
Primers for qPCR, see Table S1	This paper	N/A
Software and algorithms
FlowJo Software Version 10	Ashland, OR: Becton, Dickinson and Company; 2019	FlowJo
BD FACSDiva software Version 8.0.3	BD Biosciences	FACSDiva
Other
Vacuum pump	N/A	N/A
Desiccator	N/A	N/A
Razor blade double-sided 0.1 mm	Feather	Cat#:52-004180; https://www.labtech-em.com/em/feather-fa-10-carbon-steel-double-edge-razor-blades
5 mL polystyrene round-bottom tube with cell strainer cap	Falcon	Cat#:352235
4 laser BD FACS AriaIII cell sorter	Bioscience	N/A
1.5 mL screw-cap tubes	Sarstedt	Cat#:72.703.406
Confocal microscope	N/A	N/A
Mortar and pestle	N/A	N/A
Bioruptor Plus sonication device	Diagenode	Cat#:B01020001
Refrigerated centrifuge	N/A	N/A
Qubit fluorometer	Thermo Fisher Scientific	N/A
LightCycler 480 Instrument II	Roche	Cat#:05015243001